To confirm the involvement of glycolysis in the glucose effect, iodoacetic acid was used to inhibit glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity. rhamnosus GG survival in acidic conditions occurred only in the presence of sugars that it could metabolize efficiently. Further work with neomycin-resistant mutants that exhibited 38% to 48% of the F 0F 1-ATPase activity of the parent confirmed this, as the survival in the presence of glucose of these mutants decreased 3 × 10 6-fold compared with the survival of the wild type (which had a viability of 8.02 log 10 CFU ml −1). Addition of N′, N′-dicyclohexylcarbodiimide to simulated gastric juice caused survival to collapse, which was indicative of a prominent role in inhibition of F 0F 1-ATPase. The mechanisms behind the protective effect of glucose were investigated. rhamnosus GG survival from 6.4 to 8 log 10 CFU ml −1 in simulated gastric juice. The presence of glucose at concentrations from 1 to 19.4 mM enhanced L. Comparative analysis with other Lactobacillus strains revealed that enhanced survival was apparent in all strains, but at different pH values. Further work with dilute HCl confirmed that glucose was the sole component responsible. It was found that the presence of 19.4 mM glucose resulted in up to 6-log 10-enhanced survival following 90 min of exposure. rhamnosus GG survival was analyzed in simulated gastric juice at pH 2.0. In this study, the effect of glucose on L. Compatible with InMotion Autosampler to automate measurement and cleaning of up to 303 samples.Lactobacillus rhamnosus GG is an industrially significant probiotic strain with proven health benefits.Capture electrode calibration and results with LabX software, designed for advanced data management and 21CFR part 11 regulatory compliance.Intelligent Sensor Management (ISM) electrodes capture and save calibration data, which is then automatically recognized by the meter once connected.Features a PEEK plastic shaft for harsh environments and requires no maintenance.For use with METTLER TOLEDO SevenExcellence, SevenCompact and portable meters as well as other brands. Mettler Toledo Expert Series pH and pH/ATC Combination Electrodes with open junction puts the reference electrode in direct contact with the measured medium, eliminating the possibility of contamination or blockage. Ideal for critical samples such as suspensions or samples of unknown composition.
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